Network plot of the top 5 biological processes enriched for among genes regulated by GSDMD-KO in hyperoxia were illustrated in Supplemental Fig. 6C. Overall, these findings suggest that GSDMD deficiency prevents hyperoxia-induced ROP not only by reducing cell death but also by modulating inflammatory response, tissue remodeling, and vascular and visual developmental pathways. This evidence concerns the gene GSDMD and retinopathy of prematurity.