In order to determine whether HSF1-mediated protective autophagy and protective effect are related to Atg10 in endotoxemia, cotransfection of pcDNA3.1-HSF1 with siRNA-ATG10 or siRNA-HSF1 with pcDNA3.1-ATG10 was performed in RAW264.7 cells. This evidence concerns the gene ATG10 and serum lipopolysaccharide activity.