To do this, we engineered Her2-dependent primary tumor cells [36, 43–45] derived from the MMTV-rtTA;TetO-Her2 inducible GEM model mice [35] to either overexpress Paqr8 or delete Paqr8 using CRISPR-cas9 (Additional file 1, 11: Fig. S1, S11). The gene discussed is PAQR8; the disease is neoplasm.