To investigate the role of CD24 in regulating the macrophage-mediated immune response in OS, we treated bone marrow-derived macrophages (BMDMs) with IL-4 to generate M2-like macrophages and cocultured these less phagocytic macrophages with the GFP+ K7M2 osteosarcoma cell line for 36 h. This evidence concerns the gene CD24 and osteosarcoma.