ATF4 and cancer: Transcription factor (TF) motifs, including CEBPB (+), FOSB (+), SAP30 (+) and ATF4 (+), were significantly upregulated in CNV high cancer cells, while IRF3 (+), ETV7 (+), STAT1 (+) and IRF7 (+) were downregulated (Fig. S5c), indicating promising new regulatory networks driven by TFs in OS cells.