To further investigate centromeric CENP-A epialleles in human cell lines, we compared CENP-A position in HeLa (cervical cancer), PD-NC4 (fibroblast), RPE-1 (retinal pigment epithelium), and HuRef (lymphoblastoid cell line [Levy et al, 2007]) cells when aligned to the T2T assembly, using new (PD-NC4) and previously published CENP-A ChIP-seq ([Nechemia-Arbely et al, 2019] for HeLa; [Logsdon et al, 2021] for CHM13; [Henikoff et al, 2015] for HuRef) and CUT&RUN ([Dumont et al, 2020] for RPE-1) datasets. The gene discussed is CENPA; the disease is cervical cancer.