To evaluate the possible impact of the immune system cells, which could be infiltrated in the intestinal biopsies, interfering with the intestinal biopsy results, PACSIN2 levels were analyzed and compared in six samples of intestinal organoids and in six whole blood samples of IBD pediatric patients; no statistically significant differences in the PACSIN2 amount were detected (Fig S5A). This evidence concerns the gene PACSIN2 and inflammatory bowel disease.