In particular, higher LC3-II levels and lower SQSTM1/P62 were detected after PACSIN2 KD in both cell lines; consistently, recent results on HeLa cells showed higher LC3-II levels after PACSIN1 KO, indicating a role of PACSIN1 as a negative modulator of autophagy (Oe et al, 2022); moreover, a similar approach was used in a previous study on osteosarcoma U2OS cells and MEF cells transfected with siRNA for silencing BAP31, an ER membrane protein acting as autophagic negative modulator, that showed a higher amount of LC3-II protein expression level and LC3-GFP punctae (Machihara & Namba, 2019). This evidence concerns the gene PACSIN1 and osteosarcoma.