In conclusion, our first pan-cancer analysis of PTBP1 demonstrated a statistical correlation between the expression of PTBP1 and clinical prognosis, protein phosphorylation, immune cell infiltration, tumor mutation burden, and microsatellite instability across multiple tumors, contributing to the elucidation of the role of PTBP1 in tumorigenesis from multiple perspectives. The gene discussed is PTBP1; the disease is cancer.