To further evaluate stroke‐induced transcriptional changes of miR‐21 beyond OGD, we carried out GRO‐sequencing of mouse cortical neurons exposed to the excitatory amino acid glutamate mimicking stroke‐related glutamate excitotoxicity and detected glutamate‐induced acute transcriptional changes of several miRNAs (Figure 1D). Here, CXCL1 is linked to stroke disorder.