For instance, a recent scRNA-Seq study of a de novo GBM mouse model with human EGFR overexpression and loss of Cdkn2a and Pten identified four clusters of macrophages, including one cluster of perivascular immunosuppressive macrophages with high expression of Cd163 and Mrc1 and three clusters of microglia (57). The gene discussed is PTEN; the disease is glioblastoma.