As a key transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2) is in charge of oxidative and ER stresses, we found that alisol B (15, 30, and 60 mg/kg) could activate Nrf2 signaling pathway via upregulating expression levels of HO-1, GCLC, GCLM, NQO-1, and Nrf2, and downregulating Keap1 mRNA and expression levels (Figure 4H-J) in Cis-induced AKI mice. This evidence concerns the gene GCLM and acute kidney injury.