In contrast to previous investigations in colorectal and prostate cancer cells 19, Vimentin was inhibited after NDRG1 silencing in MDA-MB-231 and MDA-MB-436 cells treated with TGFβ1, which was not seen in SUM159 and BT549 (Fig. 3E). This evidence concerns the gene NDRG1 and Familial prostate cancer.