HAND1 and metastatic malignant neoplasm in the lymph nodes: In univariate analysis, HAND1 expression (p<0.001), age (p=0.012), histopathological grading (p=0.047), depth of invasion (p<0.001), lymph node metastasis (p<0.001), distant metastasis (p<0.001), and TNM stage (p<0.001) were significantly associated with OS in GC patients.