To characterize transcriptomic dysregulation in a human model of HNRNPU-related disorder, we first generated two isogenic mutant lines using the publicly available PGP1 induced pluripotent stem cell (iPSC) line (RRID:CVCL_F182) with a 1 bp duplication (‘D11’) and a 10 bp deletion (‘M20’), respectively (Figure 1). This evidence concerns the gene CD44 and developmental and epileptic encephalopathy, 54.