To assess the prevalence of PD-L1 and CD47 co-expression on tumor cells, 96 human tumor tissues (24 tissues for each cancer type including breast cancer, ovarian cancer, lung cancer and bladder cancer) were evaluated by multiplex fluorescent immunohistochemistry (mIHC) staining on tumor tissue microarray (TMA) slides. The gene discussed is CD274; the disease is urinary bladder carcinoma.