Shown in Fig. 2h and k, we observed a better performance of serum PRM1 in diagnosing early-staged CRC from BD and HCs in both cohorts: AUC = 0.932 in test cohort (95% CI [0.893–0.971]), AUC = 0.75 in the validation cohort (95% CI [0.686–0.813]). The gene discussed is PRM1; the disease is colorectal carcinoma.