In this study, we displayed that serum LncRNA-PAX8-AS1, LAIR-2 mRNA and its protein distinguished all hypothyroid patients from healthy controls with moderate to high sensitivity and specificity, and were distinctively expressed between clinical hypothyroid patients and healthy controls or subclinical hypothyroidism with high accuracy, sensitivity, and specificity, implying that they could be used as potential biomarkers for early and new diagnosis of hypothyroidism. Here, LAIR2 is linked to hypothyroidism.