ACSL4 and glioma: On the other hand, overexpression of ACSL4 was shown to increase the levels of ferroptosis markers, including 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, and 15-HETE (86), indicating a key role of ACSL4 in regulating ferroptosis and proliferation of glioma cells (89).