LEP and obesity due to melanocortin 4 receptor deficiency: Interestingly, the cell line DSG-BC-2 derived from a patient with obesity presented a 15% increase in proliferation when treated with 100 nM E2, while in DSG-BC-1, breast cancer cells isolated from a patient with normal BMI, pretreated with leptin, led to a 15% decrease in proliferation when stimulated with 100 nM E2.