NOTCH1 mutation did not occur in ACC of the parotid gland, accounting for 9% of ACC of the submandibular gland; meanwhile, mutations in SPEN (a negative regulator of the Notch pathway) did not appear in ACC of the submandibular gland, and the mutation frequency in ACC of the parotid gland was 11%. The gene discussed is NOTCH1; the disease is adrenal cortex carcinoma.