Both AF488-EGFHi and AF488-WT λ adhered to the HT-29 cells and accumulated so as to be resistant to rigorous washing in a time-dependent manner (Figure 4), indicating that both native and EGF-decorated phages could infiltrate the outer cellular layers of these dense CRC spheroids as with the fibroblastic stroma model. This evidence concerns the gene EGF and colorectal carcinoma.