TSPAN1 and primary biliary cholangitis: Immunohistochemical staining was performed in liver tissues of AIH (n = 66), PBC (n = 24), CHB (n = 21), and HC (n = 7), the numbers of TSPAN1+ cells were significantly increased in AIH compared to PBC (P < 0.0001), CHB (P < 0.0001), and HC (P < 0.0001) (Figure 1A).