Moreover, during T. gondii infections, the positivity for IgM antibody is proposed to be a marker of acute infection due to the occurrence of IgM antibody within days to a couple of weeks, and IgG antibodies are often interpreted as rising to protective levels after infection and remaining detectable for years, while the positivity for both IgG and IgM antibodies resulted is generally considered to indicate chronic reactivated cases (Kimbita et al., 2001; Döşkaya et al., 2014; Dhakal et al., 2015). This evidence concerns the gene CD40LG and infection.