The rSAG1 + rGRA7 positivity in IgM antibody tests indicates that the animal is suffering from acute toxoplasmosis caused by tachyzoites; rBAG1 + rGRA7 positivity indicates that the animal is being infected and is in the acute stage of infection caused by bradyzoites; rSAG1 + rGRA7 + rBAG1 indicates that the animal is suffering from acute toxoplasmosis caused by sporozoites, tachyzoites or bradyzoites. This evidence concerns the gene CD40LG and infection.