HTT and Huntington disease: To assess the transcriptional cell states of striatal and cortical mouse astrocytes within a well-established system that recapitulates many transcriptome changes in human HD tissue,30 we carried out snRNA-seq analysis of striatal and cortical brain regions from the rapidly progressing R6/2 HD model, generated through the transgenic overexpression of the first exon of human HTT, and non-transgenic (NT) mice at ages 8 weeks (symptomatic) and 12 weeks (highly symptomatic)31,32 (Figures 3A and 4A).