While muscarinic and DA receptors antagonists applied under baseline conditions (without optogenetic stimulation) did not alter sEPSCs IEIs (Supplementary Figure 3, D2-MSNs, Atr: p = 0.29, Atr + Mec: p = 0.11, Sul + SCH: p = 0.20), blocking nicotine receptors with mecamylamine significantly increased IEIs (Supplementary Figure 3, D2-MSNs Mec, Mann–Whiteny U = 21, p = 0.0387, Mann–Whitney test). This evidence concerns the gene ATR and spindle cell hemangioma.