In this study, we verified the high positive rate of S-100 and SOX in schwannomas and identified several immunohistochemical markers that could be used as potential adjunctive diagnostic markers, such as Ki-67 and β-Catenin, which had high positive rates, and SDH8, Vimentin, H3K27Me3, and Fli-1, which stained a small number of cases but achieved 100% positive rate, and perhaps they can be tested more in future immunohistochemistry to obtain more data. The gene discussed is SDHAF4; the disease is schwannoma.