TGF-β1 was used for directly stimulating the HSC-T6 cells to establish the in vitro liver fibrosis model, while LPS was employed to induce the RAW264.7 cells to establish the in vitro inflammation model as these are currently the relatively mature experimental modeling methods available for the cellular level (Dewidar et al., 2019) (Zhou et al., 2021). This evidence concerns the gene TGFB1 and Hepatic fibrosis.