SHBG and polycystic ovary syndrome: One of the main clinical features of PCOS patients is HA, manifested as elevated serum testosterone (T), free T, LH, LH/follicule-stimulating hormone (FSH) ratio, and SHBG levels (Rosenfield and Ehrmann, 2016; Saleem and Rizvi, 2017; Zhang et al., 2019; Zhang et al., 2020; Ding et al., 2021); similar changes were observed in steroid levels in rats with DHEA or letrozole-induced PCOS (Jahan et al., 2018; Poojary et al., 2022).