Our study investigates and compares how OCA and Fer-1, alone or in combination, affects liver histology, lipid accumulation, and hepatic Kupffer and stellate cell (HSC) activation as well as the impacts of these drugs on the gut microbiota and liver lipidomics in a mouse model of NASH induced by amylin liver NASH (AMLN) diet. This evidence concerns the gene IAPP and metabolic dysfunction-associated steatohepatitis.