AIFM1 and cancer: Finally, combination M + E + F increased the levels of AIF, P62, MLKL, p-MLKL, RIP3, and p-RIP3 in HCT116 cells (p < .05) but not in HDF cells (Figure 6a-b), suggesting that autophagy and necroptosis levels were also increased in the treated cancer cells (Figures 6a-b).