CTCF and neoplasm: The results highlighted the relative activation of EZH2 and CTCF in CSC-like cells (Fig. 3i), which correlated with tumor cell stemness, proliferation, and drug resistance.25,26 Moreover, clusters in the same differentiation branch shared similar TF activation, whereas those in different branches exhibited different TF activation, demonstrating that the transcriptional programs in the three differentiation trajectories are different (Fig. 3i, j).