For AFP‐negative HCC detection in the training and validation sets, the immunodiagnostic model of the six TAAbs provided sensitivity of 52.8% and 36.6%, specificity of 88.6% and 90.3% with the AUC of 0.831 and 0.834 for the identification of HCC from NC, respectively (Fig. 2B,E). This evidence concerns the gene AFP and hepatocellular carcinoma.