TIMP2 and neoplasm: In this study we created transient and stable TIMP-2 knocked down transfectants in the OVCAR5 cell line by both the siRNA and CRISPR/Cas9 methods, respectively, and systematically studied how a reduction in TIMP-2 expression affects proliferation, migration/invasion, chemosensitivity, spheroid formation (capacity of cells to form cell aggregates in a floating condition), and tumour progression and overall survival in a mouse xenotransplantation model.