We observed a significant correlation between SPP1 expression and markers of CD8 + T cell (CD8A, CD8B), T cell (CD3D, CD3E, CD2), monocyte (CD86, CD115), TAM (CCL2, IL10), M1 macrophage (IRF5, COX2), M2 macrophage (CD163, VSIG4, MS4A4A), neutrophils (CD11b, CCR7), NK cell (KIR2DL3, KIR2DL4, KIR3DL1, KIR2DS4), DC (HLA-DPB1, HLA-DRA, HLA-DPA1, BCDA-1, CD11c), Th1 (T-bet, STAT4, IFN-γ,TNF-α), Th2 (GATA3, STAT6,STAT5A,IL13), Tfh (BCL6,IL21), Th17 (STAT3,IL17A), Treg (TGFβ, FOXP3, CCR8, STAT5B), T cell exhaustion (PD-1,CTLA4,LAG3,TIM-3,GZMB) in ovarian cancer (Supplementary table 4). Here, IL21 is linked to ovarian cancer.