Lastly, the somatic mutation landscape identified TP53, KMT2C, FLG and PIK3CA as the most frequently mutated genes, together with the presence of ESR1 mutations in metastases from four individuals with estrogen receptor-positive (ER+) BC AF94, AER2, AD9I and AD9E (Fig. 1e). This evidence concerns the gene TP53 and breast cancer.