We examined the levels of AR protein in the three different TNBC cell lines (BT549, MDA-MB-453, and SUM159PT) and compared these to AR protein in the ER+/AR+ MCF7 breast cancer cell line by 1D western blots normalizing the AR band intensity to that of GAPDH as a loading control (Fig 1A). This evidence concerns the gene ESR1 and breast carcinoma.