Moreover, we examined the levels of dephosphorylated (active) cofilin and ADF (if present) and their phosphorylated forms in extracts of the three TNBC cell lines and in a rat mammary tumor cell line (MTLn3) which we previously characterized as expressing both ADF and cofilin by 2D western blots [64] (Fig 2A). This evidence concerns the gene CFL1 and breast cancer.