AKR1B10 and hepatocellular carcinoma: ELISA was used to measure AKR1B10 expression levels in serum in four studies [19, 20, 22, 23], and the pooled sensitivity and specificity for diagnosing HCC using AKR1B10 expression levels measured by ELISA were 0.80 (95% CI: 0.69–0.90) and 0.85 (95% CI: 0.73–0.94), respectively (Fig 4).