In conclusion, this study comprehensively analyzed the expression, clinicopathological feature correlation, prognostic significance, promoter methylation, immune infiltration, genetic alteration and mutation, and enrichment pathways of six-USPs, namely, USP10, USP14, USP18, USP32, USP33, and USP39, in PDAC and identified the tumor-promoting role of six-USPs in PDAC using bioinformatic analysis. The gene discussed is USP33; the disease is neoplasm.