Inhibition of TLR4/MyD88/NF-κB signaling in vivo by calycosin or TLR-4 inhibitor TAK-242 reduced the expression of various pro-inflammatory factors and promoted bone formation, effectively alleviating osteonecrosis in animals with SONFH and in bisphosphonate-related osteonecrosis of the jaw (33, 61, 66). The gene discussed is TLR4; the disease is osteonecrosis.