No differences were noted in the expression of checkpoint receptors including PD-1, PD-L1, CTLA-4, TIGIT or ICOS in CD4+ and CD8+ T cells between tumor-bearing Shp2f/fand Shp2f/fLysMCre (Supplementary Fig. 3) or in the numbers and activation of Treg cell in dLNs (Supplementary Fig. 4a,b). This evidence concerns the gene ICOS and neoplasm.