The consistent anti-inflammatory protection in liver in an overnutrition model in CD53 KO mice in vivo (Fig. 2) and the robust protection against TNFα-induced cytokine gene expression in CD53 KO isolated hepatocytes (Fig. 3) led us to examine how CD53-deficient hepatocytes respond to combined metabolic and inflammatory duress, as is seen in NASH. Here, TNF is linked to metabolic dysfunction-associated steatohepatitis.