At 10w of age, Nkcc1βKO mice showed reduced hepatic Insr expression/signaling (Fig 3A and 3B), mild focal liver steatosis (Fig 5F) and reduced hepatic glycogen stores (S5G and S5H Fig) considered early metabolic manifestations of deficient insulin-mediated responses in vivo [98, 99]. Here, INS is linked to fatty liver disease.