Apart from pathogenic or likely pathogenic findings, 41 rare VUS (Table 3) in 325 stroke-related candidate genes (Supplementary Table S1) were detected in 39 probands, including eight novel variants: ABCA1 p.H1223P, ACTA2 p.N298S, COL5A1 p.P930R, FBN1 p.Q514E, FBN1 p.K1052T, GP1BA p.P437*, RNF213 p.N1631Efs*34, and TSC1 p.P397del. The gene discussed is COL5A1; the disease is stroke disorder.