HOTAIR and breast carcinoma: We confirmed HOTAIR induction in this breast cancer model after Dox treatment using qRT-PCR, and found that HOTAIR transcript expression in PyMT mice containing the iHOT construct (iHOT+ Dox+ mice) was several hundred-fold higher compared to untreated iHOT mice (iHOT+ Dox− mice) or control mice which did not contain the iHOT construct (iHOT− mice) (Figure 2B).