Cells were harvested 4 days after transfection to extract total RNA and perform qRT-PCR for RPS26. Based on our previous experience on RPS19 downregulation (Aspesi et al., 2014) (and unpublished data), we knew that a strong silencing of the RP transcript is required in cell models to mimic the RP haploinsufficiency observed in DBA patients. This evidence concerns the gene RPS26 and Diamond-Blackfan anemia.