Our experimental work revealed that BNIP3, SLC38A5, CKMT2, CXCL11, SLC5A3, and S100A3 were expressed at higher levels, whereas PGM1 was expressed at lower levels in osteosarcoma cells compared with normal osteoblasts, highlighting the significance of the above genes in the occurrence and development of osteosarcoma. The gene discussed is BNIP3; the disease is osteosarcoma.