Our results confirmed that BNIP3, SLC38A5, CKMT2, CXCL11, SLC5A3, and S100A3 displayed higher expression, while PGM1 showed lower expression in Saos-2 and 143B cells compared with hFOB1.19 cells (Figures 8A–E), indicating that the above signature genes might participate in the progression of osteosarcoma. This evidence concerns the gene S100A3 and osteosarcoma.