Age of surgery, multifetation, weight on admission, preoperative leukocytes, albumin and C-reaction protein, operation time, meconium ileus, intestinal perforation, pulmonary infection, mesenteric dysplasia, and number of anastomosis were initially excluded (P > 0.1). This evidence concerns the gene ALB and intestinal obstruction in the newborn due to guanylate cyclase 2C deficiency.