CEBPB and major depressive disorder: To assess the impact of C/EBPβ on HFD-elicited depression-like behaviors, we bred WT(C/EBPβ+/+) and C/EBPβ heterozygous knockout (C/EBPβ+/−) mice and treated them with HFD or chow diet for 12 weeks, then evaluated a series of depression-related behaviors and molecular component levels (Figure 3A).