To further examine the effect of C/EBPβ in depression, we treated neuronal human C/EBPβ transgenic mice (Thy1-C/EBPβ Tg mice) and their littermate WT mice with HFD or chow diet for only 2 weeks before performing depression associated behaviors and molecular tests (Figure 4A). This evidence concerns the gene CEBPB and major depressive disorder.