DSG1 and pemphigus: This method requires the generation of a cell monolayer; administration of different stimuli, such as IgG fractions prepared from pemphigus patients' sera, or monoclonal antibody directed against Dsg3 (i.e., AK23) or other engineered anti‐Dsg3 and/or Dsg1 antibodies (scFv), Dsc3; dispase‐based detachment of cell monolayers from the cell culture dish; application of mechanical stress by standardized pipetting for monolayer fragmentation; and quantification of fragments (Figure 5).