Immunohistochemical staining revealed that CK7, CK19, E-cadherin, p53, Ki-67, and vimentin were positive in ICC-X1 cells, and a high degree of similarity was observed in the expression CK7, CK19, E-cadherin, p53, Ki-67, and vimentin in primary tumor tissues, transplanted tumors, ICC-X1 cells, and organoids (Fig. 5B–G). This evidence concerns the gene MKI67 and intrahepatic cholangiocarcinoma.