The results of the present study showed that the levels of NT-proBNP, HMGB1, and ACTA in the study group were higher than that in the control group, while the levels of 25-(OH) D was lower than that in the control group (P < 0.001), suggesting that biological factors have a prompt role in the disease of HIE. This evidence concerns the gene HMGB1 and perinatal asphyxia.